The shotgun approach does not require knowledge of physical mapping of the clone fragments, but rather a robust computer assembly program to join the pieces of random fragments into a single, whole-genome sequence.
The shotgun approach does not require knowledge of physical mapping of the clone fragments, but rather a robust computer assembly program to join the pieces of random fragments into a single, whole-genome sequence. Correct Answer True
Generally, the genome has to be redundantly sequenced in such a way that the overall length of the fragments covers the entire genome multiple times. This is designed to minimize sequencing errors and ensure correct assembly of a contiguous sequence. Overlapping sequences with an overall length of six to ten times the genome size are normally obtained for this purpose.
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Feb 20, 2025