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Clustal is a progressive multiple alignment program available either as a stand-alone or on-line program.

Clustal is a progressive multiple alignment program available either as a stand-alone or on-line program. Correct Answer True

Probably the most well-known progressive alignment program is Clustal. The stand-alone program, which runs on UNIX and Macintosh, has two variants, Clustal W and Clustal X. The W version provides a simple text-based interface and the X version provides a more user-friendly graphical interface.

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Like other alignment programs, CLUSTAL uses a null score for opening a gap in a sequence alignment and a penalty for extending the gap by one residue.
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The major drawback of the progressive and iterative alignment strategies is that they are largely global alignment based and may therefore fail to recognize conserved domains and motifs among highly divergent sequences of varying lengths.
Progressive alignment methods use the dynamic programming method to build an MSA starting with the most related sequences and then progressively adding less related sequences or groups of sequences to the initial alignment.