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Which step is performed after the directed mutagenesis of the gene?

Which step is performed after the directed mutagenesis of the gene? Correct Answer Expression

First, the DNA sequence is synthesized either by total synthesis of the gene or directed mutagenesis of an existing cloned gene. Then, the novel gene is introduced into a suitable expression system and the gene product is purified and biochemically characterized.

Related Questions

An electronic device rearranges numbers step-by-step in a particular order according to set of rules. The device stops when the final result is obtained.
Input: 84 15 35 04 18 96 62 08
Step 1: 96 84 15 35 04 18 62 08
Step 2: 96 84 62 15 35 04 18 08
Step 3: 96 84 62 35 15 04 18 08
Step 4: 96 84 62 35 18 15 04 08
Step 5: 96 84 62 35 18 15 08 04
And Step 5 is the last step for this input. Now, find out an appropriate step in the following question following the above rule. Question:
Input: 16 09 24 28 15 04
What will be the third step for this input ?
An electronic device when fed with the numbers, rearranges them in a particular order following certain rules. The following is a step-by-step process of rearrangement for the given input of numbers. Input :- 85 16 36 04 19 97 63 09 Step I :- 97 85 16 36 04 19 63 09 Step II :- 97 85 63 16 36 04 19 09 Step III :- 97 85 63 36 16 04 19 09 Step IV :- 97 85 63 36 19 16 04 09 Step V :- 97 85 63 36 19 16 09 04 (for the given input step V is the last step). Which of the following will be the last step for the given input ? Input :- 03 31 43 22 11 09
A device rearranges numbers step by step in a particular order according to a set of rules. The device stops when the final result is obtained. Step V is the final for this device. Input: 86 17 35 03 20 99 64 08 Step I: 99 86 17 35 03 20 64 08 Step II: 99 86 64 17 35 03 20 08 Step III: 99 86 64 35 17 03 20 08 Step IV: 99 86 64 35 20 17 03 08 Step V: 99 86 64 35 20 17 08 03 Study the above arrangement carefully and answer this question. Which one of the following would be the last step for the input below? Input: 02 30 40 20 11 08 
A word arrangement machine, When given an input line of words, rearranges it in every step following a certain rule. Following is an illustration of an input line of words and various steps of rearrangement.
Input: gone are take enough brought station
Step 1: take gone are enough brought station
Step 2: take are gone enough brought station
Step 3: take are station gone enough brought
Step 4: take are station brought gone enough
And Step 4 is the last step for this input. Now, find out an appropriate step in the following question following the above rule. Question:
Input: car on star quick demand fat
What will be the third step for this input ?
A word arranging device rearranges words step-by-step in a particular order according to set of rules. The device stops when the final result is obtained.
Input: but going for crept te light sir
Step 1: crept but going for te light sir
Step 2: crept going light but for te sir
Step 3: crept going light but for sir te
And Step 3 is the last step for this input. Now, find out an appropriate step in the following question following the above rules. Question:
Input: more fight cats cough sough acts idea
What will be the last step for this input ?
Which of the following step is performed before site-directed mutagenesis?
Which of the following statements about gene therapy are correct?
P. Affected individuals, but not their progeny, can be cured through germline gene therapy.
Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy.
R. Affected individuals, but not their progeny, can be cured through somatic gene therapy.
S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.
Refer to the figure given below. AB, CD and EF are three parallel paths. A person starts from AB to reach EF by moving in four steps - moving from AB to O1 in step 1, from O1 to CD in step 2, from CD to O2 in step 3 and from O2 to EF in step 4. If he takes a curved path in one step, he cannot take a curved path in the next step. In how many ways the person can reach EF from AB?
In a lake, there are 10 steps labelled using alphabets from A to J. Starting from step A, every minute a frog jumps to the 4th step from where it started - that is from the step A it would go to the step E and from E it would go to the step I and from I it would go to C etc. Where would the frog be at the 60th minute if it starts at the step A ?
Which of the following is not a step involved in site directed mutagenesis using a hypothetical example?