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Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
You transcribe a gene and obtain mRNA. To detect which strand is transcribed you replicate each strand in vitro and use the newly synthesized strand for hybridization with mRNA. If mRNA hybridize with the newly replicated strand from 1st strand then the gene transcribed was in ________
Consider the following statements regarding chain elongation during DNA synthesis and choose the correct answer:
1. The separated strands are kept open by DNA binding proteins (DBPs)
2. A DNA chain is initiated on the 3'- OH end of the primer
3. Okazaki fragments are synthesized on one strand; there is continuous DNA synthesis in the other strand
4. Okazaki fragments are joined by endonuclease
Who were the first to suggest that one strand of DNA might act as a template for the synthesis of its complementary strand?
A : T ratio in one strand of DNA duplex is 2, what will be the ratio in the complementary strand
The sequence can also be verified by making an oligonucleotide primer complementary to the distal part of the readable sequence and using it to obtain the sequence of the complementary strand on the original DNA template.
Watson and Crick’s suggestion of the complementary strand synthesis taking one of the parent strand as template was proposed in _________
Base sequence of each parental strand considered to synthesis new complementary strand.
Consider the following statements about DNA Sequencing: 1. DNA Sequencing is used to detect the presence of a complementary sequence by hybridization with a nucleic acid sample. 2. DNA sequencing is the process of determining the nucleic acid sequence - the order of nucleotides in DNA. Which of the above statements is/are incorrect?
De-novo methylation is done in the newly synthesized DNA strand at opposite positions of the methylated sites on the parent strand.