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Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase
Identify the correct statements.
P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction.
Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid.
R. T4 polynucleotide kinase is used for labeling 3' end of DNA.
S. Baculovirus (Autographa californica) can be used as an insect expression vector.
Which of the following statements are correct? 1. The DNA Technology (Use and Application) Regulation Bill, 2019 provides for the regulation of use of DNA technology for establishing the identity of certain persons. 2. DNA is made up of 26 pairs of chromosomes and provides instructions for building an entire organism and proteins. 3. DNA is composed of two chains, which coil around each other to form a double helix carrying the genetic instructions used in the growth. 4. Under the Bill, DNA profiling is allowed only in respect of criminal matters. Select the correct answer using the code given below:
Which of the following statements about DNA is/are correct? 1) DNA is the hereditary material of all living organisms. 2) All segments of DNA code for the synthesis of proteins. 3) Nuclear DNA is double helical with two nucleotide chains that run anti-parallel. 4) DNA is also found in mitochondria. Select the correct answer using the code given below:
Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because
Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:
Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
Consider the following statements : 1. Nucleic acids are responsible for the transfer of characters from parents to offspring. 2. Covalent bonds bind the nitrogenous bases of the two separate polynucleotide strands to make double-stranded DNA. Which of the above statements is/are correct?
Polynucleotide chain of DNA contains