Garima is a bright fair girl but orphan by birth. One she got much disturbed after learning that a couple had claimed her to be their daughter.

Question

Garima is a bright fair girl but orphan by birth. One she got much disturbed after learning that a couple had claimed her to be their daughter.

Garima is a bright fair girl but orphan by birth. One she got much disturbed after learning that a couple had claimed her to be their daughter. Although it is good but she felt that she did not belong to them. One of her close friends Nisha who was very much caring for her contacted the orphanage executives to find out the validity of the claim of the couple through DNA profiling.

Answer the following questions on the basis of above information:

(i) How DNA profiling can be made use of for testing the validity of parenthood claim of the couple.

(ii) Which branch of biology was this Programme associated with ?

(iii) What values were shown by Nisha.

Monjur Alahi

6 views

2025-01-04 04:42

1 Answers

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Salim Ahmed Kawsar · Accepted Answer · 2023-02-05T15:29:48+06:00

(i) The couple's claim of being parent of Galima by DNA finger printing technique the main steps of which are as follows :

(a) DNA is extracted from certain cell of the body of Garima and the parents claiming for parenthood and is amplified using PCR.

(b) This DNA is cut in fragments with the help of specific restriction enzymes which are then subjected to get electrophoresis.

(c) Double stranded DNA is then split into single stranded DNA.

(d) The separated DNA sequences are later transferred from gel nylon membrane (Southern blotting).

(e) The nylon sheet is immersed in a bath where radioactive synthetic DNA segments called as probes or markers are added and hybridized With VNTR.

(f) The hybridized DNA fragments are detected by autoradiography. They are observed as dark bands on X-Ray film which represent the DNA finger prints or DNA profiles.

(g) If the bands of Garima's DNA match will the DNA print of claiming couple, the claim of the couple of being her parents is true otherwise not.

(ii) HGP is closely associated with the development of new era of biology called as Bioinformatics.

(iii) The values like humility, sense of responsibility awareness and tendency tb help the needy persons were shown by Nisha a close friend of Garima.