During in vitro synthesis of DNA,
During in vitro synthesis of DNA, a researcher used 2’, 3’–dideoxy cytidine triphosphate as raw nucleotide in place of2’-deoxy cytidine triphosphate other conditionsremainingas standard. Will furtherpolymerisation of DNA continue upto the end ornot? Explain.
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Further polymerisation would not occur, as the 3’OH on sugar is not there to add a newnucleotide for forming ester bond.
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