During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide

Monjur Alahi

During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine triphosphate other conditions remaining as standard. Will further polymerisation of DNA continue upto the end or not? Explain.


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Salim Ahmed Kawsar

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Further polymerisation would not occur, as the 3’ OH on sugar is not there to add a new nucleotide for forming ester bond.

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