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In enzymology, a glutamate—ethylamine ligase is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are ATP, L-glutamate, and ethylamine, whereas its 3 products are ADP, phosphate, and N5-ethyl-L-glutamine.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-ammonia ligases. The systematic name of this enzyme class is L-glutamate:ethylamine ligase. Other names in common use include N5-ethyl-L-glutamine synthetase, theanine synthetase, and N5-ethylglutamine synthetase.

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