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In enzymology, a phosphoenolpyruvate-protein phosphotransferase is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are phosphoenolpyruvate and protein histidine, whereas its two products are pyruvate and protein Npi-phospho-L-histidine.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups with a nitrogenous group as acceptor. This enzyme participates in phosphotransferase system.

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