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In enzymology, a trimethylamine dehydrogenase is an enzyme that catalyzes the chemical reaction

The 3 substrates of this enzyme are trimethylamine, H2O, and electron-transferring flavoprotein, whereas its 3 products are dimethylamine, formaldehyde, and reduced electron-transferring flavoprotein.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with a flavin as acceptor. The systematic name of this enzyme class is trimethylamine:electron-transferring flavoprotein oxidoreductase. This enzyme participates in methane metabolism.

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