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In enzymology, a glycosphingolipid deacylase is an enzyme that catalyzes a chemical reaction that cleaves gangliosides and neutral glycosphingolipids, releasing fatty acids to form the lyso-derivatives.

This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is glycosphingolipid amidohydrolase. This enzyme is also called glycosphingolipid ceramide deacylase.

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