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In enzymology, an uridine nucleosidase is an enzyme that catalyzes the chemical reaction

Thus, the two substrates of this enzyme are uridine and H2O, whereas its two products are D-ribose and uracil.

This enzyme belongs to the family of hydrolases, specifically those glycosylases that hydrolyse N-glycosyl compounds. The systematic name of this enzyme class is uridine ribohydrolase. This enzyme is also called uridine hydrolase. This enzyme participates in pyrimidine metabolism.

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