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Thymidine kinase is an enzyme, a phosphotransferase : 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, EC 2.7.1.21 that catalyzes the reaction:

Thd + ATP → TMP + ADP

where Thd is thymidine, ATP is adenosine 5’-triphosphate, TMP is thymidine 5’-phosphate and ADP is adenosine 5’-diphosphate. In clinical chemistry it has been suggested as a proliferation marker for prognosis, verification of diagnosis, control of treatment and follow-up of malignant disease. It is used mainly in relation to hematological malignancies but the developments of more sensitive assays have stimulated investigations for its use in relation to solid tumors.

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