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Consider the following statements regarding Tissue culture. 1. The capacity to generate a whole plant from any cell/explant is called micropropagation. 2. The method of producing thousands of plants through tissue culture is called totipotency. Which of the statements given above is/are correct?

Consider the following statements regarding Tissue culture. 1. The capacity to generate a whole plant from any cell/explant is called micropropagation. 2. The method of producing thousands of plants through tissue culture is called totipotency. Which of the statements given above is/are correct? Correct Answer Neither 1 nor 2

The correct answer is Neither 1 nor 2.

Key Points

Tissue culture:

  • It was learned by scientists, during the 1950s, that whole plants could be regenerated from explants, i.e., any part of a plant taken out and grown in a test tube, under sterile conditions in special nutrient media.
  • This capacity to generate a whole plant from any cell/explant is called totipotency. Hence, Statement 2 is incorrect.
  • It is important to stress here that the nutrient medium must provide a carbon source such as sucrose and also inorganic salts, vitamins, amino acids, and growth regulators like auxins, cytokinins, etc.
  • By application of these methods, it is possible to achieve propagation of a large number of plants in very short durations.
  • This method of producing thousands of plants through tissue culture is called micropropagation. Hence, Statement 1 is incorrect.
    • Each of these plants will be genetically identical to the original plant from which they were grown, i.e. they are some clones.
    • Many important food plants like tomato, banana, apple, etc. have been produced on a commercial scale using this method.
  • Another important application of the method is the recovery of healthy plants from diseased plants.
    • Even if the plant is infected with a virus, the meristem (apical and axillary) is free of virus.
    • Hence, one can remove the meristem and grow it in vitro to obtain virus-free plants.
    • Scientists have succeeded in culturing meristems of banana, sugarcane, potato, etc. 

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