A low Km value points out a low affinity of an enzyme towards its substrate.

A low Km value points out a low affinity of an enzyme towards its substrate. Correct Answer False

Km is defined as the substrate concentration which, results in the half-maximal velocity of the enzyme reaction. The value of Km varies from one enzyme to other and also affected by certain conditions like pH, temperature, ionic strength, nature of solvent etc. Also, the values of Km can be different for the mutant forms of enzyme, isoenzyme and even for different substrates catalyzed by the same enzyme. A low value Km specifies, a high affinity of an enzyme towards its substrate i.e., at even low substrate concentration of substrate an enzyme can initiate the reaction and a high Km points out a low affinity of an enzyme towards its substrate. Hence the above statement is false.
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