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Consider the following statements regarding genetic code and choose the correct answer:
1. The genetic code is degenerate
2. In all amino acids the first two bases are the same in all the codons that represent a particular amino acid
3. The number of codons an amino acid can claim is proportional to the frequency of the amino acid in proteins
4. Amino acids with codons ending in U or A also have codons ending in C or G respectively
In a batch process, the reaction takes place in the presence of an acid medium. The acid is drained from the reaction vessel at the rate of 15ml/s as a result of the density difference of the acid from the reacting component. To avoid wastage of acid, it is recycled to an acid tank which has 1000 L capacity. The acid drained from the reaction vessel, picks up 50 g/L solids from the reactor. Acid is fed once again to the process from acid tank. When the process is started, the acid is almost pure in the tank as a result of filtration. As the reaction proceeds, acid in the tank gets more and more contaminated with the solids. The concentration of the solids should not exceed 100 g/L from the process point of view. The batch time is 16h. Estimate whether the concentration of the solids will exceed 100g/L during the batch reaction.
Analysis of a hexapeptide using enzymatic cleavage reveals the following result:
• Amino acid composition of the peptide is: 2R, A, V, S, Y
• Trypsin digestion yields two fragments and the compositions are: (R, A, V) and (R, S, Y)
• Chymotrypsin digestion yields two fragments and the compositions are: (A, R, V, Y) and (R, S)
• Digestion with carboxypeptidase A yields no cleavage product.
Given: Trypsin cleaves at carboxyl side of R. Chymotrypsin cleaves at carboxyl side of Y. Carboxypeptidase A cleaves at amino side of the C-terminal amino acid (except R and K) of the peptide.
The correct amino acid sequence of the peptide is:
An example of polar but uncharged amino acid is ___________
PROTPARS counts the minimum number of mutations to change a codon for the first amino acid into a codon for the second amino acid, but only scores those mutations in the mutational path that actually change the amino acid.
Often, protein to be expressed is fused with histidine and it is called as histidine tags. For their purification, matrix containing ______ is used.
You design a plasmid with a gene for Histidine synthesis and a fragment containing ARS. You introduce it yeast culture and try to grow then in Histidine deficit media. What will be your observation?
When an uncharged body is placed near a charged body, then uncharged body
Name the hormone which is synthesized from histidine amino acid?
Three molecular ionic states, P-R are possible for the amino acid histidine. Identify the correct choice of pH values, respectively for the observation of the ionic states P-R.
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