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In the strategy devised by Maniatis, how many restriction enzymes were used?
Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately
A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site.
Which set of the following events occurs during the elongation step of translation?
P. Attachment of mRNA with the smaller subunit of ribosome
Q. Loading of correct aminoacyl-tRNA into the A site.
R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site.
S. Dissociation of the ribosomal subunits.
T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome.
Which of the following statements about Restriction enzymes is NOT correct? 1. Exonucleases make cuts at specific positions within the DNA 2. Endonucleases removes nucleotides from the ends of the DNA
In a survey of 25 students, it was found that 15 had taken Mathematics, 12 had taken Physics and 11 had taken Chemistry, 5 had taken Mathematics and Chemistry, 9 had taken Mathematics and Physics, 4 had taken Physics and Chemistry and 3 had taken all the three subjects. Find the number of students who had taken both Physics and Chemistry but not Mathematics.
In the following question the 1st and the last part of the sentence/passage are numbered 1 and 6. The rest of the sentence/ passage is split into four parts and named P, Q, R and S. These four parts are not given in their proper order. Read the sentence/passage and find out which of the five combinations is correct. 1 - Henchard's wife acted for the best, but she had involved herself in difficulties. P - But she had refrained.  Q - A hundred times she had been upon the point of telling her daughter Elizabeth-Jane the true story of her life, the tragical crisis of which had been the transaction at Weydon Fair, when she was not much older than the girl now beside her. R - An innocent maiden had thus grown up in the belief that the relations between the genial sailor and her mother were the ordinary ones that they had always appeared to be. S - The risk of endangering a child's strong affection by disturbing ideas which had grown with her growth was to Mrs. Henchard too fearful a thing to contemplate. 2 - It had seemed, indeed folly to think of making Elizabeth-Jane wise.   
The government approved the net neutrality which implies that telecom service providers must treat all internet traffic equally, without any regard to the type, origin or destination of the content. Thus, enabling access to the internet as unrestricted and non-discriminatory. Which of the following is the cause of the statement? I: Restriction of net neutrality allows the internet access service provider to block, degrade, slow down or grant speeds to particular content. II: Restriction of net neutrality affects those who depend on the internet. III: Restriction of net neutrality could result in the discrimination against specific services or websites by the internet access service providers.