To form a circular plasmid for dispersed genes, intramolecular ___________ is required.

To form a circular plasmid for dispersed genes, intramolecular ___________ is required. Correct Answer Ligation

When different gene segments are assembled in a linear order then it is necessary to undertake intermolecular ligation. To form a circular plasmid requires intramolecular ligation.
Bissoy MCQ

Related Questions

"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Consider the following statements and choose the correct answer:
1. In the phenomenon of linkage and crossing over, the genes are arranged in a linear order in a chromosome
2. The linkage groups in an organism are equivalent to the number of chromosome pairs
3. The chances of crossing over are more between two closely placed genes as compared to the genes placed in far proximity
4. The position of genes is very definite in the chromatid
Consider the following statements regarding the theory of linkage and choose the correct answer:
1. Genes are arranged in a linear fashion in the chromosomes
2. Genes that show linkage are situated in the chromosome
3. Linked genes remain in their original combination during the course of inheritance
4. The genes which are widely separated show strong linkage, while those closely located, have more chances to get separated by crossing over
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase
A suit, a trouser, and a shirt are to be stitched from a cloth. The cloth required for the suit is the average of cloth required for trousers and shirt. The length of the cloth for suit is 1.5 times the cloth required for the shirt. The length of cloth required for shirt is 5 times the even prime number. Which of the following is/are correct? A. Cloth required for trousers is 20 m B. Average length of cloth required for all the dresses = Cloth required for suit C. Initially, the cloth was 45 m D. Cloth required for shirt is 15 m
If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
Once a plasmid has incorporated specific genes, such as the gene coding for the antibiotic ampicillin, into its genome, the plasmid may be cloned by
The following sentences form a paragraph. These four parts are numbered as P, Q, R and S. They are not given in their proper order. Read the sentences and choose the alternative that arranges them in correct order. P. The hypothesis was proposed as an explanation for the increased prevalence of type-2 diabetes in certain populations by American geneticist James V. Neel. Q. Thrifty genes refers to a scientific hypothesis which states that obesity in some human beings can be explained by the existence of a ‘thrifty gene’ that aided the storage of excess energy as fat. R. The same genes, however, turned into a biological liability that led to obesity in some people. S. According to the hypothesis, certain genes, by helping in the storage of energy as fat, might have enabled human ancestors live through periods of famine, thus enhancing their chances of survival.
Which one of the following is NOT a colloidal solution with dispersed phase-gas and dispersed medium-solid?