Positive Bacteria MCQ

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• The integrative vectors for P. aeruginosa have their origin of replication derived from __________
  ক

  RP4

  খ

  PMB1

  গ

  Lambda

  ঘ

  M13
• For chromosomal integration selective ____________ has to be maintained.
  ক

  Pressure

  খ

  Temperature

  গ

  Size

  ঘ

  Host range
• Genomic DNA and plasmid DNA differ in their extent of ____________
  ক

  Temperature

  খ

  Weight

  গ

  Supercoiling

  ঘ

  Hybridization
• Luciferase is a ______________
  ক

  Promoter

  খ

  Inducer

  গ

  Reporter

  ঘ

  Suppressor
• For proper ___________ analysis of a cloned gene, it must be present on the chromosome.
  ক

  Translational

  খ

  Transcriptional

  গ

  Transversal

  ঘ

  Replicative
• The high GC content of Streptomycetes affects the frequency of restriction sites.
  ক

  True

  খ

  False
• Cloning in Streptomyces is done to analyze _____________ synthesis.
  ক

  Enzyme

  খ

  Antibiotic

  গ

  Promoter

  ঘ

  Protein
• With phage vectors, plaques can be obtained in __________
  ক

  One night

  খ

  Fortnight

  গ

  One week

  ঘ

  2-3 days
• What is the average insert size of phiC31 vector?
  ক

  4 kb

  খ

  8 kb

  গ

  10 kb

  ঘ

  12 kb
• PhiC31 is a ___________ phage of Streptomyces.
  ক

  Toxic

  খ

  Transient

  গ

  Temperate

  ঘ

  Infectious
• SLP1 and pSAM2 are examples of _______________ plasmids.
  ক

  E. coli

  খ

  Streptomyces

  গ

  Agrobacterium

  ঘ

  Yeast
• SCP is a progenitor of low-copy-number vectors.
  ক

  True

  খ

  False
• SCP2 is a ______ plasmid.
  ক

  Toxic

  খ

  Antibiotic

  গ

  Sex

  ঘ

  Integrative
• Most of the vectors used with Streptomyces are derivatives of ____________
  ক

  Bacteriophages

  খ

  B. subtilis

  গ

  E. coli

  ঘ

  Pseudomonas
• GC content in gram-positive bacteria is not more than _______ percent.
  ক

  30

  খ

  50

  গ

  70

  ঘ

  90
• Tandem sites are used for the recognition of _________
  ক

  Alpha factors

  খ

  Sigma factors

  গ

  Beta factors

  ঘ

  Gamma factors
• Which of the following helps in achieving higher plasmid DNA take up?
  ক

  Polyethylene glycol

  খ

  Lactose

  গ

  Sucrose

  ঘ

  Galactose
• Transformation, transfection and conjugation are ways of introducing ________ into the cell.
  ক

  DNA

  খ

  Toxins

  গ

  Enzymes

  ঘ

  Host
• Streptomyces genes are difficult to express in _________
  ক

  Mammals

  খ

  E.coli

  গ

  Bacillus

  ঘ

  Streptococcus
• Promoters in Streptomyces can be _______ during gene cloning.
  ক

  Lost

  খ

  Decreased

  গ

  Increased

  ঘ

  Inactivated
• For the construction of gene libraries, which of the following sequence is not used?
  ক

  BamHI

  খ

  BglII

  গ

  BelI

  ঘ

  EcoR1
• AT-rich recognition sites are rare and this is useful if _______ fragments of DNA are desired.
  ক

  Small

  খ

  Mid-sized

  গ

  Large-sized

  ঘ

  Mixed
• Streptomycete DNA has a G+C content of _____ percent.
  ক

  10

  খ

  50

  গ

  70

  ঘ

  90
• Streptomyces coelicolor is _________ for genetic studies.
  ক

  Incapable

  খ

  Model

  গ

  Not suitable

  ঘ

  New
• A large number of antibiotics are made from the genus Streptomyces.
  ক

  True

  খ

  False
• To form a circular plasmid for dispersed genes, intramolecular ___________ is required.
  ক

  Attraction

  খ

  Distraction

  গ

  Ligation

  ঘ

  Restriction
• When B. subtilis is transformed, ordered assembly of ________ genes is facilitated.
  ক

  Scarce

  খ

  Regular

  গ

  Dispersed

  ঘ

  Interspersed
• ________ is used to relieve the production of mutants in B. subtilis.
  ক

  Lactose

  খ

  Nisin

  গ

  Galactose

  ঘ

  IPTG
• The two mutants generated during the process of cloning are null mutation and ________ mutation.
  ক

  Combinational

  খ

  Conventional

  গ

  Conditional

  ঘ

  Rational
• In the process of cloning with a B. subtilis vector, _________ mutants are simultaneously generated.
  ক

  1

  খ

  2

  গ

  3

  ঘ

  4