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"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
How can conjugative and non-conjugative plasmids be differentiated?
If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?
The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________
Ti plasmid is the only known plasmid of plant.
A Yeast integrative plasmid cannot replicate as a plasmid inside the host?
In the binary vector strategy, the sizes of the two vectors used are _____ for bigger plasmid and ______ for smaller T-DNA plasmid.
What is the basic difference between Ri plasmid and Ti plasmid of Agrobacterium?
__________ demonstrated a plasmid-induced immune response using mice by inoculating with a plasmid expressing human growth hormone which elicited antibodies instead of faltering growth.
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase