Related Questions

"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Which antibiotic resistance is present in pBR322?
A manufacturer of TV wants to make a profit of Tk. 300,000 on sale of 200 TV sets. It costs Tk. 10,000 each to make the first 100 TV sets and Tk. 7,500 each to make TV sets after the first 100 sets. What should be the selling price of each TV sets in Taka?
If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded?
Once a plasmid has incorporated specific genes, such as the gene coding for the antibiotic ampicillin, into its genome, the plasmid may be cloned by
Who were the scientists who discovered the plasmid pBR322?
The par region from a plasmid such as Psc101 can be cloned into Pbr322. What is the function of this region?
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
How many unique cleavage sites are located within the antibiotic resistance determinants of plasmid RSF1010?
Name the plasmid which makes host cell resistance to the antibiotic.