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The DNA to be cloned must be cleaved along with the vector and with the same restriction enzymes.

The DNA to be cloned must be cleaved along with the vector and with the same restriction enzymes. Correct Answer True

Large DNA molecules have to be broken down to produce fragments small enough to be carried by the vector. Most of the cloning vectors are very inefficient in carrying DNA more than 8kb in length.

Related Questions

Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally
Consider a system described by ẋ = Ax + Bu y = Cx + Du The system is completely output controllable if and only if Where: x = State vector (n-vector) u = Control vector (r-vector) y = Output vector (m-vector) A = n × n matrix B = n × r matrix C = m × n matrix D = m × r matrix
In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme.
The restriction endonuclease Eco52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco52I?
The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?
h,J,K,L,M,N and O are the only legislators eligible to vote on Bills 1,2 and 3, each of which will be passed if at least four legislators eligible vote in favor of it. each legislator must vote on all three bills; on abstentions are possible. The following is known : *H must vote against all three bills; each of the other legislators must vote in favor of at least one bill and against at lest one bill. *j must vote against Bills 1. *O Must vote against Bills 2 and 3. * L must the same way that K dose on all three bills.*N must vote the same way that O dose on all three bills. which of the following must vote in favor of Bill 1 ?
h,J,K,L,M,N and O are the only legislators eligible to vote on Bills 1,2 and 3, each of which will be passed if at least four legislators eligible vote in favor of it. each legislator must vote on all three bills; on abstentions are possible. The following is known : *H must vote against all three bills; each of the other legislators must vote in favor of at least one bill and against at lest one bill. *j must vote against Bills 1. *O Must vote against Bills 2 and 3. * L must the same way that K dose on all three bills.*N must vote the same way that O dose on all three bills. If any bill is to be passed,which of the following must be among the legislators who vote in favor of it?
h,J,K,L,M,N and O are the only legislators eligible to vote on Bills 1,2 and 3, each of which will be passed if at least four legislators eligible vote in favor of it. each legislator must vote on all three bills; on abstentions are possible. The following is known : *H must vote against all three bills; each of the other legislators must vote in favor of at least one bill and against at lest one bill. *j must vote against Bills 1. *O Must vote against Bills 2 and 3. * L must the same way that K dose on all three bills.*N must vote the same way that O dose on all three bills. If M votes the same way that O dose on all three bills, which of the following can be determined ?
To constitute a matter of res judicata which of the following conditions must concur?
1. The matter directly and substantially in issue in the subsequent suit or issue must be the same matter which was directly and substantially in issue either actually (section 11, explanation III) or constructively (section 11, explanation IV) in the former suit
2. The former suit must have been a suit between the same parties under whom they or any of them claim. Explanation VI of Section 11 must be read with this condition
3. The parties as aforesaid must have litigated under the same title in the former suit
4. The court which decided the former suit must have been a court competent to try the subsequent suit of the suit in which such issue has been subsequently raised. Explanation II of section 11 is to be read with condition
5. The matter directly and substantially in issue in the subsequent suit must have been heard and finally decided by the court in the first suit. Explanation V of section 11 is to be read with this condition