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"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
Which of the following feature(s) facilitate cloning into a vector? 1. Origin of replication 2. Selectable marker 3. Cloning sites
The addition of two binary variables A and B results in a SUM and a CARRY output. Consider the following expressions for SUM and CARRY outputs. (a) SUM \( = A \cdot B + \;\overline {AB} \) (b) SUM = A⋅B̅ + A̅ ⋅ B (c) CARRY = A⋅B (d) CARRY = A + B Which of the following expressions are correct?
A candidate takes a test and attempts all the 100 questions in it. While any correct answer fetches 1 mark, the wrong answers are penalised as follows; one-tenth of the questions carry 1/10 negative marks each, one-fifth of the questions carry 1/5 negative marks each and the rest of the questions carry 1⁄2 a negative mark each. Unattempted questions carry no marks. What is the difference between the maximum and the minimum marks that he can score? 
In the binary vector strategy, the sizes of the two vectors used are _____ for bigger plasmid and ______ for smaller T-DNA plasmid.
As in the determination of the various unknown forces in the free body diagram involves the use of vectors. So one of the vector law is commutative law and it is valid for the cross product of two vectors. (Commutative law: PxQ = QxP; for two vectors P and Q)
What is the major difference between cloning vectors and primary vectors?
All the series of cloning vectors developed from PAMB1 carry repE and copF which is a __________
Which one of the following four addresses is NOT EXACTLY same as the one given below? Christine Smith 6, Kutuzovskaya st., Platforma Kutuzovskaya, Podolsk, Moscow region, Russia (i) Christine Smith 6, Kutuzovskaya st., Platforma Kutuzovskava, Podolsk, Moscow region, Russia (ii) Christine Smith 6, Kutuzovskaya st., Platforma Kutuzovskaya, Podolsk, Moscow region, Russia (iii) Christine Smith 6, Kutuzovskaya st., Platforma Kutuzovskaya, Podolsk, Moscow region, Russia (iv) Christine Smith 6, Kutuzovskaya st., Platforma Kutuzovskaya, Podolsk, Moscow region, Russia