Bissoy.com
Doctors Medicines MCQs Q&A

Which of the following is responsible for the conversion of covalently closed circular DNA to supercoiled DNA of the plasmid?

Which of the following is responsible for the conversion of covalently closed circular DNA to supercoiled DNA of the plasmid? Correct Answer DNA Gyrase

If both strands of DNA are intact, it is in a relaxed form called CCC DNA, if only one strand is intact it is called open circular DNA. When isolated from cells covalently closed circles (CCC DNA) often have a deficiency of turns in the double helix, such that they have a supercoiled configuration.

Related Questions

Which one of the following is the correct sequence of various stages of operating cycle of a manufacturing company?  1. Raw material conversion period, work-in-progress conversion period, Finished goods conversion period, Receivables conversion period 2. Raw material conversion period, work-in-progress conversion period, Creditors payment period, Receivables conversion period 3. Creditor payment period. Raw material conversion period, Receivables conversion period, work-in- progress conversion period 4. Raw material conversion period, Finished goods conversion period, work-in- progress conversion period. Receivables conversion period 
Which of the forms of plasmid DNA can be differentiated by electrophoresis on agarose gel? i. Covalently closed ii. Open circular iii. Supercoiled iv. Linear
The schematic of a plasmid with a gap in one of the strands is shown below:
Which of the following enzyme(s) is/are required to fill the gap and generate a covalently closed circular plasmid?
P. DNA ligase
Q. Alkaline phosphatase
R. DNA polymerase
S. Polynucleotide kinase
"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
The mixing vat in a factory receives liquid ingredients through six separate value--- labeled R.S.T.U,Y and Z -- each of which has exactly two settings: open and closed.The mixing vat operator must ensure that each valve is set open or closed according to the following conditions: If T is open, both S and Z must be closed, R and Z cannot both be closed at the same time; If Y is closed, Z must also be closed;S and U cannot both be open at the same time. If the maximum number of valves that can be closed at the same time are closed which of the following must true?
The mixing vat in a factory receives liquid ingredients through six separate value--- labeled R.S.T.U,Y and Z -- each of which has exactly two settings: open and closed.The mixing vat operator must ensure that each valve is set open or closed according to the following conditions: If T is open, both S and Z must be closed, R and Z cannot both be closed at the same time; If Y is closed, Z must also be closed;S and U cannot both be open at the same time. If R is closed and U is open, which of the following must be true?
The mixing vat in a factory receives liquid ingredients through six separate value--- labeled R.S.T.U,Y and Z -- each of which has exactly two settings: open and closed.The mixing vat operator must ensure that each valve is set open or closed according to the following conditions: If T is open, both S and Z must be closed, R and Z cannot both be closed at the same time; If Y is closed, Z must also be closed;S and U cannot both be open at the same time. If Z is open, which of the following must be true?
The mixing vat in a factory receives liquid ingredients through six separate value--- labeled R.S.T.U,Y and Z -- each of which has exactly two settings: open and closed.The mixing vat operator must ensure that each valve is set open or closed according to the following conditions: If T is open, both S and Z must be closed, R and Z cannot both be closed at the same time; If Y is closed, Z must also be closed;S and U cannot both be open at the same time. Which of the following, if given to the mixing vat operator as an instruction, would not determine the setting of any other valve ?
Topological winding number of a 2.0 kb covalently closed circular DNA was found to be 191 with a writhing number of -4. Hence, its linking number and the number of base pair per turn when the molecule is laid flat on the surface is . . . . . . . . and . . . . . . . ., respectively.
Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally