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"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments.
Plasmid + HindIII → 1200bp and 3800bp
Plasmid + BamHI → 5000bp
Plasmid + EcoRI → 2500bp
The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"
Consider a system described by ẋ = Ax + Bu y = Cx + Du The system is completely output controllable if and only if Where: x = State vector (n-vector) u = Control vector (r-vector) y = Output vector (m-vector) A = n × n matrix B = n × r matrix C = m × n matrix D = m × r matrix
A vector (cloning vector) is a carrier DNA molecule to which the human DNA fragment is attached in DNA transfer the vectors used from smallest to largest is:-
Which of the following feature(s) facilitate cloning into a vector? 1. Origin of replication 2. Selectable marker 3. Cloning sites
Which of the following is a versatile cloning vehicle used most frequently as a cloning vector?
A plasmid cloning vector should contain all of the following sequences except
Consider the following three statements about link state and distance vector routing protocols, for a large network with 500 network nodes and 4000 links. [S1] The computational overhead in link state protocols is higher than in distance vector protocols. [S2] A distance vector protocol (with split horizon) avoids persistent routing loops, but not a link state protocol. [S3] After a topology change, a link state protocol will converge faster than a distance vector protocol. Which one of the following is correct about S1, S2, and S3?
Consider the following statements and choose the correct answer:
In order to be a useful vector, a plasmid:
1. Must possess the genes essential for replication of DNA
2. Must carry, at least, one gene that can be used as a marker for the selection of the plasmid in the transformed cell
3. Must include, at least, one gene recognizable by its product or activity, which possesses within it a cutting site for a restriction enzyme
4. In the rep region must contain a cutting site for the restriction enzyme to be used
A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is
To be a cloning vector, a plasmid does not require