In an experiment of determining the order of gene in bacteria we use HFR which is gal+, Lac+, Ara+ and His+. If the recipient is negative for all these genes, and we selectively choose Gal + and get the pattern- 100 % recipients are Gal + 30% are His+ 55% are Lac + And only 5% are Ara+ What would be our inference?
In an experiment of determining the order of gene in bacteria we use HFR which is gal+, Lac+, Ara+ and His+. If the recipient is negative for all these genes, and we selectively choose Gal + and get the pattern- 100 % recipients are Gal + 30% are His+ 55% are Lac + And only 5% are Ara+ What would be our inference? Correct Answer Sequence of gene is Gal—Lac—His—Ara
While the HFR could incorporate in different positions by selecting one gene we are setting a start site for checking. Now the gene order agrees with the percent incorporation as the farther genes have a lower probability of incorporation.
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Feb 20, 2025