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You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result?

You designed an antibody against phosphorylated CTD of RNA polymerase II. Then you use a DNA fragment with lac operon and try to immunoprecipitate the RNA pol II. What will be the expected result? Correct Answer No RNA pol II will precipitate

Lac operon is a prokaryotic gene not recognized by eukaryotic RNA polymerase. Thus it will not be transcribing. Non-Transcribing RNA pol II doesn’t have phosphorylated residue in CTD, so it can’t be immunoprecipitated with the given antibody.

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