Bissoy.com
Doctors Medicines MCQs Q&A

While studying the transcripts in vitro you observe that other than the longer transcripts there are some short stretches of RNA oligomer produced as well. You repeat the experiment several times but these oligomers seem to persist. What is the possible explanation?

While studying the transcripts in vitro you observe that other than the longer transcripts there are some short stretches of RNA oligomer produced as well. You repeat the experiment several times but these oligomers seem to persist. What is the possible explanation? Correct Answer Experimental artifact

These short oligomers are abortive transcripts produced by the RNA polymerase before it leaves the promoter. There is no lagging strand in RNA and since the experiment is repeated several times with caution the other justifications are rules out.

Related Questions

Simplify the value of $$\frac{{{\text{0}}{\text{.9}} \times {\text{0}}{\text{.9}} \times {\text{0}}{\text{.9 + 0}}{\text{.2}} \times {\text{0}}{\text{.2}} \times {\text{0}}{\text{.2 + 0}}{\text{.3}} \times {\text{0}}{\text{.3}} \times {\text{0}}{\text{.3}} - {\text{3}} \times 0.9 \times {\text{0}}{\text{.2}} \times {\text{0}}{\text{.3}}}}{{{\text{0}}{\text{.9}} \times {\text{0}}{\text{.9 + 0}}{\text{.2}} \times {\text{0}}{\text{.2 + 0}}{\text{.3}} \times {\text{0}}{\text{.3}} - 0.9 \times {\text{0}}{\text{.2}} - {\text{0}}{\text{.2}} \times {\text{0}}{\text{.3}} - 0.3 \times 0.9}} = ?$$
$$\frac{{38 \times 38 \times 38 + 34 \times 34 \times 34 + 28 \times 28 \times 28 - 38 \times 34 \times 84}}{{38 \times 38 + 34 \times 34 + 28 \times 28 - 38 \times 34 - 34 \times 28 - 38 \times 28}}$$            is equal to = ?
To constitute a matter of res judicata which of the following conditions must concur?
1. The matter directly and substantially in issue in the subsequent suit or issue must be the same matter which was directly and substantially in issue either actually (section 11, explanation III) or constructively (section 11, explanation IV) in the former suit
2. The former suit must have been a suit between the same parties under whom they or any of them claim. Explanation VI of Section 11 must be read with this condition
3. The parties as aforesaid must have litigated under the same title in the former suit
4. The court which decided the former suit must have been a court competent to try the subsequent suit of the suit in which such issue has been subsequently raised. Explanation II of section 11 is to be read with condition
5. The matter directly and substantially in issue in the subsequent suit must have been heard and finally decided by the court in the first suit. Explanation V of section 11 is to be read with this condition
S1: If you want to do well in your examinations you need to be able to think for yourself which means not just following the guide-books but write what you think yourself.
P: That will not help much.
Q: Few if any students do this.
R: By discussing things with other students, with your teachers, and with any intelligent people you meet you will find you can pick up a lot of new ideas but it is not good first accepting these ideas, swallowing them undigested and then repeating them in the examination.
S: At first you will find it difficult but if you go on trying you will find clear independent thought becomes easier.
S6: If however you turn these ideas over in our mind accepting those which you agree with and fitting them into your stock of knowledge and rejecting the others you may get somewhere.

The Proper sequence should be:
Given below are two statements. One is labelled as Assertion (A) and the other is labelled as Reason (R).  Assertion (A): In the case of short-run cost behaviour, MC curve intersects AC curve at its minimum  Reason (R): While AC continues to decrease, MC begins to rise. Also, when AC is at its minimum, it is neither increasing nor decreasing. It is constant when AC is constant, it is equal to MC at the point of intersection  Choose the correct option from those below  1. Both A) and R) true and R) is the correct explanation of A) 2. Both A) and R) true but R) is NOT the correct explanation of A) 3. A) is true but R) is false 4. A) is false but R) is true 
In an experiment, you are trying to pull down the site where guanyl transferase binds by co-immunoprecipitation. You run a gel and autoradiograph to see a band denoting GSK-RNA pol II (phosphorylated). What will you see when you perform the same experiment with overexpression of GSK in one setup and overexpression of unlabelled GSK-RNA pol II in the other setup?
Oligomers are often obligate complexes meaning that the free-energy cost of dissociation is high and they exist as oligomers under physiological conditions.
We all observe old wells which have dried up. Our elders say that about 25-30 years ago there was sufficient water in the wells, but now these have completely dried up. Select from the following the possible correct reasons for drying up of water in the wells. A. The lakes (ponds) in which rainwater used to collect are no longer there. B. The soil around the trees, well and nearby area is now covered with coal tar/cement. C. No one uses wells as there are taps in every house. D. A number of electric motor driven pumps have come up to draw underground water.
Small quantities of radiolabelled RNA can be produced by translation in vitro which is done by transcription in vitro.
Which is the longer: P is equal to Q, R is shorter than P, S is longer than R. T is longer than Q, is longer then S.