How many prokaryotic DNA polymerases have 5’->3’ proofreading activity?

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

A

1, 2, 3 and 4 are true

B

Only 2 and 4 are true

C

Oniy 2, 3 and 4 are true

D

Only 1 and 2 are true

Polymerases are available with proof reading activity. Which of the following are the characteristics of these types of polymerases?

A

They add A residue at 3’ end

B

They are obtained from Thermococcus litoralis

C

They can’t be obtained from archaebacteria

D

The marine bacteria from which they are obtained grow at temperatures lower than that of Thermus aquatics

You wanted to find out the importance of the DNA polymerases. So, you mutated the polymerases one by one and checked for survival. In which of these mutant cases will you see least survival rate?

A

Alpha

B

Beta

C

Delta

D

Eta

Which is the most processive of prokaryotic DNA polymerases?

A

pol I

B

pol II

C

pol III

D

klenow fragment

Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?

A

5’ to 3’ exonuclease activity

B

3’ to 5’ exonuclease activity

C

3’ to 5’ endonuclease activity

D

5’ to 3’ endonuclease activity

Bacterial polymerases are slower than eukaryotic polymerases.

A

True

B

False

All the RNA polymerases share 5 common subunits, which are present in all the three polymerases and are all alike. State whether the statement is true or false.

A

True

B

False

Proofreading activity existing universally in synthetase as a double sieve mechanism.

A

True

B

False

Which of the following statements are correct? 1. The DNA Technology (Use and Application) Regulation Bill, 2019 provides for the regulation of use of DNA technology for establishing the identity of certain persons. 2. DNA is made up of 26 pairs of chromosomes and provides instructions for building an entire organism and proteins. 3. DNA is composed of two chains, which coil around each other to form a double helix carrying the genetic instructions used in the growth. 4. Under the Bill, DNA profiling is allowed only in respect of criminal matters. Select the correct answer using the code given below:

A

Only 1 and 2

B

Only 2 and 3

C

Only 1 and 3

D

1, 2, 3 and 4

Which of the following statements about DNA is/are correct? 1) DNA is the hereditary material of all living organisms. 2) All segments of DNA code for the synthesis of proteins. 3) Nuclear DNA is double helical with two nucleotide chains that run anti-parallel. 4) DNA is also found in mitochondria. Select the correct answer using the code given below:

A

1, 2 and 3 only

B

3 and 4 only

C

1, 3 and 4 only

D

4 only

How many prokaryotic DNA polymerases have 5’->3’ proofreading activity?

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