The viral DNA may be incorporated into the phage head during phage assembly.

Consider the following statements regarding DNA replication and choose the correct answer:
1. The very first requirement of DNA synthesis is the presence of a template or plan along which the new strand is to be constructed
2. The second requirement is of a DNA-dependent-DNA polymerase or simply DNA polymerase enzyme
3. A DNA Pol cannot join two free nucleotides; one of them must be anchored by hydrogen bonds to a DNA strand
4. Replication takes place bidirectionally

A

1, 2, 3 and 4 are true

B

Only 2 and 4 are true

C

Oniy 2, 3 and 4 are true

D

Only 1 and 2 are true

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

A

3, 1, 2, 4, 6, 5, 7, 8

B

1, 4, 3, 2, 5, 7, 6, 8

C

1, 2, 6, 7, 5, 8, 3, 4

D

8, 6, 7, 5, 1 , 4, 2, 3

M13 filamentous phage DNA molecule is smaller than the head-and-tail lambda phage.

A

True

B

False

You create an assembly by using Visual Studio .NET. The assembly is responsible for writing and reading order entry information to and from an XML data file. The assembly also writes and reads values to and from the Windows registry while it is being consumed. The assembly will be distributed to client computers by using your company, XYZ, intranet. All client computers are configured to implement the default .NET security policy. You need to implement security in the assembly. What should you do?

A

Implement declarative security and execute the permission demand to allow access to the file system and Windows registry.

B

Implement declarative security and execute the minimum permission request to allow access to the file system and Windows registry.

C

Implement imperative security and execute the permission demand to allow access to the file system and Windows registry.

D

Implement imperative security and execute the minimum permission request to allow access to the file system and Windows registry.

Which of the following proteins are involved in initiating phage assembly and maintaining the stability of the viral particle?

A

gp III and gp VIII

B

gp VIII and gp II

C

gp VI and gp IV

D

gp VII and gp IX

Compared to DNA viral replication, RNA viral replication is more susceptible to mutation because

A

RNA replication takes place in the cytoplasm, whereas DNA replication takes place in the nucleus.

B

RNA viruses replicate much faster than DNA viruses.

C

RNA viruses use uracil instead of thymine.

D

RNA replication lacks the proofreading functions characteristic of DNA replication.

Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because

A

The sequence context at the RB defines the direction of transfer

B

The sequence context at the LB defines the direction of transfer

C

The nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand

D

The endonuclease activity of VirD2 protein allows nicking at RB

Which of the following statements about DNA is/are correct?
1. DNA is the hereditary material of all living organisms.
2. All segments of DNA code for synthesis of proteins.
3. Nuclear DNA is double helical with two nucleotide chains which run anti-parallel.
4. DNA is also found in mitochondria.
Select the correct answer:

A

1, 2 and 3

B

3 and 4

C

1, 3 and 4

D

Only 4

What is the confirmation of lambda phage DNA when it is isolated from the phage particle?

A

Supercoiled

B

Open circular

C

Linear

D

Linear duplex

Consider the following about the powers of Legislative Assembly of Punjab
1. The money bill is introduced only in the Legislative Assembly.
2. The ministers are collectively responsible to the Legislative Assembly.
3. The elected members of the Legislative Assembly form part of the electoral college which elects the President of India.
Which of the statement(s) given above is/are correct?

A

Only 1

B

2 and 3

C

1 and 3

D

All of these

The viral DNA may be incorporated into the phage head during phage assembly.

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