Which of the following is a mismatch?
A
Polymerase – Taq polymerase
B
Template – double stranded DNA
C
Primer – oligonucleotide
D
Synthesis – 5’ to 3’ direction
Correct Answer: Template – double stranded DNA
PCR uses the enzyme DNA polymerase that directs the synthesis of DNA from deoxynucleotide substrates on one stranded DNA template. DNA polymerase synthesizes DNA in a 5’→3’ direction and can add nucleotides in the 3’ end of a custom – designed oligonucleotide.