The various steps for construction of libraries are ___________ i) Fragmentation of DNA ii) Isolation of genomic DNA iii) Amplification iv) Ligation and introduction to the host v) Vector preparation The correct order of construction of libraries is (In the order of starting to ending).

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is

A

3, 1, 2, 4, 6, 5, 7, 8

B

1, 4, 3, 2, 5, 7, 6, 8

C

1, 2, 6, 7, 5, 8, 3, 4

D

8, 6, 7, 5, 1 , 4, 2, 3

The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: i) Peeling of membranes carrying away bacterial cells with it ii) Cells are lysed and denaturing of DNA is being carried out iii) Hybridization with labelled DNA iv) Placing the membrane onto plate containing recombinant cells Choose the correct sequence in which the steps are carried out (starting to ending).

A

iv)-i)-ii)-iii)

B

iv)-ii)-i)-iii)

C

i)-ii)-iii)-iv)

D

iii)-ii)-i)-iv)

Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?

A

AMP is added to the 5’ phosphate of one of the DNA molecule

B

It leads to the liberation of pyrophosphate from NAD and nicotinamide mononucleotide from ATP

C

The AMP is further displaced by an electrophilic attack

D

The AMP is further displaced by nucleophilic attack by 3’ hydroxyl of the same DNA molecule

Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor

A

P and Q

B

R and S

C

P, Q and S

D

All of these

Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.

A

Only I is true

B

Both I and II are true

C

Only II is true

D

I is true and II is false

Three cats are roaming in a zoo n such a way that when cat A takes 5 steps, B takes 6 steps and C takes 7 steps.But the 6 steps of A are equal to the 7 steps of B and 8 steps of C. what is the ratio of their speeds?

A

140:144:147

B

40:44:47

C

15:21:28

D

252:245:240

For the construction of DNA library what is the range of genomic DNA that has to be cloned into the vector?

A

Less than 100 bases

B

100 to 1000 bases

C

Less than 100 to mega bases

D

Less than 10 bases

Choose the incorrect statement for the preparation of genomic libraries.

A

The first step is the isolation of genomic DNA

B

Physical damage to the DNA should be avoided

C

If a nuclear DNA library is to be constructed, organelle DNA is to be removed

D

For the construction of organelle library, organelle DNA is purified from the nuclear DNA

These are steps taken in carrying out the PCR reaction: i) Attaching of primers by cooling ii) Denaturation of strands iii) DNA synthesis iv) Heating Which is the correct order? (Mentioned from starting to ending the reaction)

A

i)-ii)-iii)-iv)

B

ii)-i)-iii)-iv)

C

iv)-iii)-ii)-i)

D

iv)-ii)-i)-iii)

Consider the following statements regarding the general symptoms of diseases and choose the correct answer:
1. The charges in the host which serve to recognise the disease are called the signs and symptoms of the disease
2. The sign of a disease is the external appearance of some portion of the pathogen of the host
3. The symptoms are the visible effects which the host plant induces on the parasites
4. Any visible deviation on the host plant from the normal structure and function is called a symptom

A

Only 1, 2 and 4 are true

B

Only 2, 3 and 4 are true

C

Only 2 and 4 are true

D

1, 2, 3 and 4 are true

The various steps for construction of libraries are ___________ i) Fragmentation of DNA ii) Isolation of genomic DNA iii) Amplification iv) Ligation and introduction to the host v) Vector preparation The correct order of construction of libraries is (In the order of starting to ending).

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