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The various steps for construction of libraries are ___________ i) Fragmentation of DNA ii) Isolation of genomic DNA iii) Amplification iv) Ligation and introduction to the host v) Vector preparation The correct order of construction of libraries is (In the order of starting to ending).

The various steps for construction of libraries are ___________ i) Fragmentation of DNA ii) Isolation of genomic DNA iii) Amplification iv) Ligation and introduction to the host v) Vector preparation The correct order of construction of libraries is (In the order of starting to ending). Correct Answer ii)-i)-v)-iv)-iii)

Firstly, the isolation of genomic DNA is carried out. It is followed by the fragmentation of DNA. Then the vector preparation is done, which is followed by the ligation and introduction to the host. The final step is the amplification of the recombinants.

Related Questions

Steps of Recombinant DNA technology are given below.
1. Identification and isolation of the genetic material.
2. Fragmentation of DNA.
3. Obtaining the foreign gene product.
4. Downstream processing.
5. Ligation of DNA fragmentation into the vector.
6. Isolation of desired DNA fragments.
7. Amplification of gene of interest.
8. Transfer of Recombinant DNA into the host cell/organism.
The correct sequence of steps is
The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps: i) Peeling of membranes carrying away bacterial cells with it ii) Cells are lysed and denaturing of DNA is being carried out iii) Hybridization with labelled DNA iv) Placing the membrane onto plate containing recombinant cells Choose the correct sequence in which the steps are carried out (starting to ending).
Mechanism of ligation for both T4 DNA ligase and E. coli DNA ligase makes use of Adenosine Monophosphate (AMP). Which of the steps is involved in the ligation mechanism?
Amplified fragment length polymorphism (AFLP)
P. A PCR-based tool
Q. Uses restriction enzymes to cut genomic DNA
R. Involves ligation of adaptors to the sticky ends of the restriction fragments
S. Involves amplification using primers complementary to the adaptor
Consider the following statements.
I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase.
II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.
A, B and C participated in a race. A covers the same distance in 49 steps as B covers in 50 steps and C covers in 51 steps. A takes 10 steps in same time as B takes 9 steps and C takes 8 steps. Who is the winner of the race?
A man walks 60 steps in a minute when he has energy. He walks 30 steps per minute when he is tired. He has to go to a place 300 steps away. If he can walk 120 steps without getting tired, what is his average walking speed (steps per minute) for his back and forth journey if he takes rest after reaching the place and again walks 120 steps with energy.
Three cats are roaming in a zoo n such a way that when cat A takes 5 steps, B takes 6 steps and C takes 7 steps.But the 6 steps of A are equal to the 7 steps of B and 8 steps of C. what is the ratio of their speeds?
Consider a system described by ẋ = Ax + Bu y = Cx + Du The system is completely output controllable if and only if Where: x = State vector (n-vector) u = Control vector (r-vector) y = Output vector (m-vector) A = n × n matrix B = n × r matrix C = m × n matrix D = m × r matrix
In the question below are given three statements followed by three conclusions numbered I, II and III. You have to take the given statements to be true even if they seem to be at variance with commonly known facts. Read all the conclusions and then decide which of the given conclusions logically follows from the given statements disregarding commonly known facts. Statements: I. Some preparation are not good II. Only a few good are wise III. No wise is preparation Conclusions: I. Some good are not wise is a possibility II. No preparation is wise III. Some preparation are good is a possibility