By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength.

By using two oligonucleotides, we can measure the amount of quantity of DNA by measuring the amount of fluorescence. The first probe absorbs light and the second emits it at a particular wavelength. Correct Answer True

If two oilgonucleotides are used, which bind nearby to the target DNA help in quantifying the DNA. The first probe is meant for absorbing light and the second probe absorbs the light emitted by the first one and then re-emits it. DNA is quantified by measuring the fluorescence from the second probe and it is possible only when both the probes are close to each other i.e. they have bound to the location near the target DNA.
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