Consider the following techniques/phenomena: 1. Budding and grafting in fruit plants 2. Cytoplasmic male sterility 3. Gene silencing Which of the above is/are used to create transgenic crops?

Consider the following techniques/phenomena: 1. Budding and grafting in fruit plants 2. Cytoplasmic male sterility 3. Gene silencing Which of the above is/are used to create transgenic crops? Correct Answer 2 and 3

The correct answer is 2 i.e. 2 and 3 only.

  • Transgenic Crops are the crops that have their genomes modified through genetic engineering techniques either by the addition of a foreign gene or removal of a certain detrimental gene. the aim is to introduce a new trait to the plat which does not naturally occur.
  • Budding and Grafting are horticultural techniques, Grafting is the act of placing a portion of one plant (bud or scion) into or on a stem, root, or branch of another (stock) in such a way that a union will be formed and the partners will continue to grow.
    • In the budding process, a bud is taken from one plant and grown on another. it does not lead to any genetic modification. Hence, option 1 is not correct.
  • Cytoplasmic male sterility is total or partial male sterility in plants as the result of specific nuclear and mitochondrial interactions.
    • Male sterility is the failure of plants to produce pollen or male gametes.
    • The cytoplasmic male sterility systems are widely utilized in crop plants for hybrid breeding due to the convenience of controlling sterility.
  • Gene Silencing using RNA interference technology, transcriptional gene silencingvirus-induced gene silencing, has been used in horticultural techniques.
    • The double-strand RNAs present inside the cell are responsible for producing small interfering RNAs, short hairpin RNAs, and micro RNAs.

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