Bissoy.com
Doctors Medicines MCQs Q&A

Which of the following statements is true for a refractive index detector in HPLC?

Which of the following statements is true for a refractive index detector in HPLC? Correct Answer It does not respond to many solutes.

The Refractive Index Detector in HPLC does not respond to many solutes. It only detects those with Refractive Index different from the solvent.

Key Points

  • The Refractive index is the universal detector in HPLC because of its detection capabilities.
  • RI Detectors measure the refractive index of (analytes) column eluent passing through the flow cell it is based on the two principal one is deflection and another is a reflection type refractometer.
  • It is used to detect sugar, alcohol, and inorganic ions.

There are different other detectors for HPLC, these are:

  • UV/VIS and PDA Detector - These are of two types - Fixed wavelength detector, variable wavelength detector
  • Mass detector (LC-MS)
  • Fluorescence Detector
  • Electrochemical Detector
  • Conductivity Detector
  • Light Scattering Detector
  • Infrared Detector

Additional Information

  • High-Performance Liquid Chromatography is a modern analysis technique that is used in various fields like analysis of biochemical, organic, and inorganic compounds commonly found in complex samples of environmental and biological origin.
  • This technique yields perfect results in the separation, identification, and quantification of the analyte to be analyzed.
  • HPLC instrumentation includes a pump, injector, column, detector, and integrator or acquisition and display system.
  • The heart of the system is the column where separation occurs.

Related Questions

Choose the correct relation for a combination of two prisms to be achromatic. (Where for the first prism: μv = refractive index of the medium in violet light, μr = refractive index of the medium in red light A = angle of the prism; For the second prism: μ′v = refractive index of the medium in violet light, μ′r = refractive index of the medium in red light, A’ = angle of prism.)
A 4 km optical link consists of multimode step index fiber with core refractive index of 1.3 and a relative refractive index difference of 1%. Find the delay difference between the slowest and fastest modes at the fiber output.
A multimode step-index fiber has a core refractive index of 1.5 and relative refractive index difference of 1%. The length of the optical link is 6 km. Estimate the RMS pulse broadening due to intermodal dispersion on the link.
A crown glass prism with refractive index 6° is to be achromatized for red and blue color light with flint glass prism. What will be the angle of flint glass prism? (µr = 1.513, µr‘ = 1.645, µ2 = 1.523 and µ2‘ = 1.665, Where µr and µ2 are refractive indices of red and blue light respectively whereas, µr‘ and µ2‘ are refractive indices of red and blue light respectively for flint glass.)
The core refractive index should be lesser than the cladding refractive index for a coaxial cable. State True/False
When light passes from air to glass it experience change in refractive index. and due to change in refractive index the wavelength of light changes and hence speed.
A convex lens of refractive index 1.49 is immersed in a certain liquid. For the lens to act as a transparent plane sheet, what should be the refractive index of the liquid?
According to the Snell's law if an electromagnetic wave is incident in a medium (refractive index n₁) on another medium (refractive index m₂);
If a monochromatic light goes through 2 cm of glass or 2.25 cm of water, the optical path is the same. If refractive index of glass is 1.50, then what is the refractive index of water?
If light travels in a medium of Refractive Index 1.4 through a distance 250 m in a given time interval, then how much distance will it cover in refractive index 1.75?