Suspected colonies of Staphylococcus aureus when grown on Baird Parker medium shall show

Suspected colonies of Staphylococcus aureus when grown on Baird Parker medium shall show Correct Answer Coagulase activity

Suspected colonies of Staphylococcus aureus, when grown on Baird Parker medium, shall show Coagulase Activity.

Key Points

• Baird Parker with Rabbit Plasma clotting factor will be used for the detection of enzyme activity.
• Coagulase-positive organisms seem as gray to black colonies due to the tellurite reduction and as an opaque halo, because of the conversion by an enzyme from fibrinogen to protein.
• Baird Parker Agar is used for the isolation and differentiation of coagulase-positive staphylococci in food and prescribed drugs according to the Baird Parker technique.
• As a nitrogen supply for the organism, casein peptone, and meat extract are added to the medium.
• Yeast extract provides nitrogen similarly to different necessary nutrients like B-complex.
• The medium contains lithium and tellurite, which inhibit most of the contaminating microflora, whereas glycine and pyruvate enhance Staphylococci growth.
• Staphylococci will scale back tellurite to telluride, which ends in grey to the black coloration of the colonies.
• With the addition of egg yolk, the medium becomes yellow, slightly opaque. 

Additional Information Coagulase activity 

• Coagulase is a catalyst had ought to build blood clots.
• This catalyst is existing in staphylococcus aureus (S. aureus) bacteria.
• Coagulase test is used to differentiate Staphylococcus aureus (positive) that produces the catalyst coagulase, from S. epidermis and S. saprophyticus (negative) that don't produce coagulase (clumping).

Protease activity

• Protease activity is measured by imaging the activated Probody molecule binding to an antigen present in the sample.
• The method was evaluated in xenograft tumor samples using protease-specific substrates and inhibitors, and the measurements correlated with the efficacy of the respective Probody therapeutics.
• A decrease in the water activity and pH and/or temperature caused a decrease in protease activity.
• It occurred that the combined impact of temperature, water activity, and pH scale influences catalyst production considerably compared with the influence of single environmental factors.

Catalase activity

• Catalase (hydrogen peroxide/hydrogen peroxide oxidoreductase) is a very important cellular antioxidant catalyst that defends against aerophilous stress.
• It's found within the peroxisomes of most aerobic cells.
• It's vital to measure catalase levels as a result of aerophilous stress is inherent in pathological conditions like cancer, diabetes, cataracts, atherosclerosis, neurodegenerative illness, aging, and nutritionary deficiencies.
• This unit provides strategies for catalase activity measurements.