Gram stain of skin lesion

Gram Stain of Skin Lesions

A Gram stain is a laboratory test used to identify different types of bacteria from skin lesions. A sample of the lesion is taken using a cotton swab, and placed onto a slide. The sample is then stained to allow for the bacteria to become visible. It is then examined under a microscope to determine what types of bacteria are present.

Preparation

Preparation for a Gram Stain includes collecting a sample of the lesion, and mounting it onto a microscope slide. The sample must be kept dry and undamaged.

Procedure

The procedure for a Gram Stain involves smearing the sample onto the slide, and adding a few drops of crystal violet dye. After a few seconds, Gram's iodine is added, and a few seconds later, the dye is decolourized using 95% ethanol. A red safranin counterstain is then added to the slide, and the sample is then viewed under a microscope to determine what kind of bacteria is present.

Types

Gram negative bacteria are purple under the microscope, while Gram positive bacteria are pink. Examples of Gram positive bacteria include Streptococcus and Staphylococcus, while examples of Gram negative bacteria include E. coli and Salmonella.

Risks

The main risk associated with a Gram stain is an allergic reaction to the dyes used. It is important to wear gloves when performing a Gram stain, to avoid coming into contact with these substances.

Why

Gram staining is an essential tool for identifying different types of bacteria. It allows doctors to quickly detect the presence of certain bacteria, and in turn, provide the necessary treatment.

When

Gram staining may be used in the diagnosis of a variety of skin lesions, such as boils, infected cuts, and burns. It may also be used to identify the cause of an infection, such as a urinary tract infection, or an infection of the intestines.