Factor IX assay
What is Factor IX Assay?
An Factor IX assay is a laboratory test to measure the amount of Factor IX (FIX) activity in a blood sample. Factors IX assay is also referred to as FIX antigen assay, antigen-binding assay, and FIX gene sequencing. Factor IX, also known as Christmas Factor, is a protein that plays a fundamental role in the normal clotting process.
Preparation for Factor IX Assay
The blood sample is collected from the patient by venipuncture. Before the blood collection, the patient must fast for at least 8 hours and move for a minimum of 15 minutes prior to the collection so that the blood sample is not compromised. The patient should not eat, drink or smoke 2 hours prior to the collection.
Procedure for Factor IX Assay
The blood sample is collected in a sterile container which contains anticoagulant that keeps the blood from clotting. The sample is then sent to the lab where it undergoes a few procedures to measure the amount of Factor IX. The procedure involves determining the amount of antigen or protein associated with Factor IX and also measuring the activity of the enzyme from the Factor IX gene.
Types of Factor IX Assay
- ENzyme-Linked Immunosorbent Assay (ELISA) – it is a type of antigen-binding assay that is used to measure the amount of Factor IX in the blood.
- Factor IX gene sequencing – this type of Factor IX assay is used to identify the mutations in the Factor IX gene.
Risk of Factor IX Assay
There are no risks associated with the Factor IX Assay. The risk associated with the procedure is the same as a normal venipuncture.
Why perform Factor IX Assay
The Factor IX Assay is performed to diagnose and monitor bleeding disorders like Hemphilia (A and B) and Von Willibrand’s Disease. It is also used to evaluate the abnormal coagulation (clotting) caused by Factor IX deficiency, to monitor the treatment of Factor IX deficiency, and to detect biomarkers associated with deep vein thrombosis.
When is Factor IX Assay Ordered?
A Factor IX Assay is ordered by a healthcare provider when a patient is suspected of having a bleeding disorder, and the individual has a family history of clotting disorders. It is also ordered when the patient is being treated for Factor IX deficiency, and the treatment is not effective. Additionally, it may be ordered to evaluate a deep vein thrombosis or to monitor the level of factor IX.
