Culture - duodenal tissue
What is Duodenal Tissue Culture?
Duodenal tissue culture methods are used to analyze the regulatory and functional aspects of the upper part of the gastrointestinal tract. It is one of the most common forms of bioreactor technology in cell and tissue biology. This technique is used to investigate the influence of growth factors, enzymes, or hormones on the metabolism of human or animal cells. It is also used to investigate the transport of drugs or radiosensitivity of small intestine tissue.
Preparation
To perform a duodenal tissue culture, the duodenal process must be washed, emulsified, and homogenized in a solution at pH 7.4. The homogenized sample is then divided into two equal parts and is divided into both explants and cultures. The multiple explants are placed on a plastic chamber or elastic material in a humid atmosphere and contain the culture medium.
Procedure
The explants are then incubated in the medium for at least 48 hours before proceeding to the cell culture. The explants are then thoroughly washed in the medium before placing into new dishes. The cells are then cultured in cell culture dishes and a serum matrix is used for cell adhesion. The culture temperature must be maintained at 37°C and a 5% carbon dioxide atmosphere is maintained for the culture.
Types
Duodenal tissue cultures are available in two types: primary and secondary cultures. Primary cultures are derived from organ tissue samples directly. They contain a large portion of live cells which may degenerate quickly after 24 hours. Secondary cultures are derived from primary cultures that have been adapted to increased proliferation. They have longer periods of cell sustainability.
Risks
Duodenal tissue culture is a complex process and can cause various risks in the process of its application. Some of the risks include contamination of the sample due to improper washing, contamination of the medium, insufficient oxygen supply, and inadequate nutrient supply. Additionally, microbiological contamination, mycoplasma contamination, and medium evaporation can all be risks posed when utilizing this technique.
Why?
Duodenal tissue culture is used to identify the metabolic pathways in the small intestine of humans or animals. It is used for research applications in the study of small intestine physiology, pharmacology, and disease mechanisms. It is also used to investigate the transport of compounds such as drug molecules, hormones, or nutrients.
When?
Duodenal tissue culture is typically used for research purposes. However, it can also be used for clinical applications, such as tissue engineering and drug delivery. The technique can be used to study the effects of drugs or hormones on duodenal tissue in vivo or in vitro.
