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Significance:
1. Carboxylation: RuBisCO is the most abundant enzyme in the world. It is responsible for fixing carbon in the form of C0 into sugar. As a result of Carboxylation, the first stable product of carbon fixation i.e. 3- PGA is synthesized.
2. Reduction/Glycolytic reversal: NADPH2 donates electrons to 1, 3-Bisphoshoglycerate to form 3- phosphoglyceraldehyde molecules. During this process ADP and NADP are generated which are used in light reaction.
3. Regeneration of RuBP: Some 3-phosphoglyceraldehyde molecules are involved in production of glucose while others are recycled to regenerate the 5-carbon compound RuBP which used to accept new carbon molecules. Thus, regeneration of RuBP is required for Calvin cycle to run continuously.
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