Write the steps of BLAST involved in comparison of DNA sequences.

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Monjur Alahi

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2025-01-04 04:42

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Salim Ahmed Kawsar · Accepted Answer · 2023-02-05T15:29:48+06:00

The given sequence is compared with sequences in the database using substitution matrices that specify scores to either 'reward' a match or 'penalize' a mismatch.
Top scoring matches are ranked according to set criteria that serve to distinguish between a similarity due to ancestral relationship or due to random chance.
True matches are further examined thoroughly with other details accessible through Entrez and other tools available at NCBI.