Summarize the process by which the sequence of DNA bases in Human Genome Project was determined using the method developed by Frederick Sanger. Name a free living non-pathogenic nematode whose DNA has been completely sequenced.

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• The sequences were arranged based on some overlapping regions present in them (Alignment of these sequences was not humanly possible)
• Therefore, SQPecialized computer based programme was developed.
• These sequences were subsequently annotated and were assigned to each chromosome-
• Chromosome 1
• Caenorhabditis elegans

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