A plasmid DNA and linear DNA (both are of the same size) have one site for restriction endonuclease. When cut and separated on agarose gel electrophor

Question

A plasmid DNA and linear DNA (both are of the same size) have one site for restriction endonuclease. When cut and separated on agarose gel electrophor

A plasmid DNA and linear DNA (both are of the same size) have one site for restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band, while linear DNA shows two fragments Explain.

Monjur Alahi

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2025-01-04 04:42

1 Answers

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Salim Ahmed Kawsar · Accepted Answer · 2023-02-05T15:29:48+06:00

When plasmid DNA and a linear DNA having on e site for a restriction exndonuclease are cut and separted, plasmid shows one DNA band, while linear DNA shows two band because of difference in their basic structure.
Plasmid is a circular DNA molecule and when cut with these enzyme. it becomes linear but does not get fragmented due to presence of only one restriction site, whereas a linear DNA. molecule gets cut into two fragment.