The linking of antibiotic resistance gene with the plasmid vector became possible with
The linking of antibiotic resistance gene with the plasmid vector became possible with
(a) DNA polymerase
(b) exonucleases
(c) DNA ligase
(d) endonucleases
4 Answers
Correct option (c) DNA ligase
Explanation:
The linking of antibiotic resistance gene with the plasmid vector became possible with the enzyme DNA ligase, which acts on cut DNA molecules and joins their ends. This makes a new combination of circular autonomously replication DNA created in vitro and is known as recombinant DNA.
Correct option: (a) DNA ligase
Explanation:
The linking of antibiotic resistance gene with the plasmid vector became possible with DNA ligase. DNA ligase is an enzyme that is able to join together two portions of DNA and therefore plays an important role in DNA repair. DNA ligase is also used in recombinant DNA technology as it ensures that the foreign DNA is bound to the plasmid into which it is incorporated.