The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

Question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

(a) Non-recombinant bacteria containing β -galactosidase

(b) lnsertional inactivation of α -galactosidase in non-recombinant bacteria

(c) lnsertional inactivation of α-galactosidase in recombinant bacteria

(d) lnactivation of glycosidase enzyme in recombinant bacteria

Monjur Alahi

8 views

2025-01-04 04:42

1 Answers

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Salim Ahmed Kawsar · Accepted Answer · 2023-02-05T15:29:48+06:00

Correct option (c) lnsertional inactivation of α-galactosidase in recombinant bacteria

Explanation:

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of insertional inactivation of alpha galactosidase in recombinant bactena. Alpha galactosidase is a glycoside hydrolase enzyme that hydrolyse the terminal alpha galactosyl moieties from glycolipids and glycoprotein. It is encoded by the GLA gene. β-galactosidase is an exoglycosidase. which hydrolyses the β-glycosidic bond formed between a galactose and its organic moiety.