A sugarcane has been affected by the virus. How can a virus free cane developed from it?

Virus free sugarcane can be developed by tissue culture technique. The meristematlc regions of a  plant are generally free from virus, Healthy and virus free sugarcane plants can by developed by this techmique using meristems as explants.

Procedure : Explants obtained from the meristem apical or young axillary buds or from nodes near the stem apex  are taken and surface sterlized by 0.5% Sodium hypochlorite and  then seeded over the nutrient agar medium enriched with cytokinin under   aseptic conditions. After incubation a  few days under optimal conditions the explants microprogive rise to shoots. these shoots are cut into nodal Jegments of 2-3 cm which are then transferred into a medium containing extra auxin for promoting  root formation. This results in the development of a number of plantlets, which are then hardened and transferred to fields.