Write the steps you suggest to be undertaken to obtain a foreign-gene-product.
Write the steps you suggest to be undertaken to obtain a foreign-gene-product.
4 views
1 Answers
Recombinant DNA technology is used to obtain foreign gene product.
It involves following steps :
1. Identification and isolation of gene of interest.
2. Adding or ligating the gene of interest into suitable vector, resulting in formation of a complete recombinant DNA molecule.
3. Transfer of this recombinant DNA molecule into suitable host cell.
4. Selection of recombinants and non- recombinant cells.
5. Expression of gene of interest by culturing recombinant cells under suitable conditions.
6. Culturing of cells in bioreactors for large-scale production of desired product.
4 views
Answered