What was the challenge for production of insulin using rDNA techniques ? How did Eli Lilly produce insulin using rDNA technology ?
What was the challenge for production of insulin using rDNA techniques ? How did Eli Lilly produce insulin using rDNA technology ?
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The challenge for production of insulin using r DNA technique was getting insulin assembled into a mature form
• Prepared two DNA sequence corresponding to A and B chains of human insulin.
• introduced them in plasmids of E.coli to produce insulin chains.
• chains A and B were produced separately.
• extracted and combined by creating disulfide bonds to form human insulin
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