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An NTP binding site is a type of binding site found in nucleoside monophosphate kinases, N can be adenosine or guanosine. A P-loop is one of the structural motifs common for nucleoside triphosphate binding sites, it interacts with the bound nucleotide's phosphoryl groups. For the binding site to be able to bind a nucleotide, the nucleotide must be complex bound to Mg or Mn. Nucleotide binding will cause conformational changes in the protein because the P-loop will bend.
NTP binding sites play a role in poliovirus RNA replication. On the poliovirus RNA-dependent polymerase, also known as 3D, there are two binding sites. Both binding sites contain lysine residues; however, only the lysine at position 61 is essential for RNA sequence elongation as part of the replication process. Additionally, a method known as computational alanine mutagenesis, has been used to find the key portions within the amino acid sequences that majorly characterize a particular NTP binding site; these regions within the sequence are conserved.